Product Information

Contents: Phycoerythrin (PE) anti-mouse CD98 (4F2)
Catalog Number: 12-0981
Sizes: 50 ug, 100 ug, 200 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: RL388
Isotype: Rat IgG2a, κ

Surface staining of mouse splenocytes with FITC (left) anti-mouse CD98 (RL388) and 0.25 μg PE (right) anti-mouse CD98 (RL388). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0981	Affinity Purified anti-mouse CD98 (4F2)	N/A	N/A	FC IP
11-0981	FITC anti-mouse CD98 (4F2)	488	518	FC
12-0981	PE anti-mouse CD98 (4F2)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The RL388 monoclonal antibody reacts with mouse CD98, also known as 4F2 antigen and Ly-10. RL388 detects a disulfide-linked heterodimer complex (130 kDa) composed of a glycosylated heavy (86 kDa) and a nonglycosylated light (39 kDa) subunits. CD98 is expressed on the surface of activated lymphocytes and is involved in cell activation and calcium flux events.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The RL388 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The RL388 antibody has been tested by flow cytometric analysis of activated and resting mouse splenocyte cell suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

MacDonald HR, Lees RK, Bron C. 1985. Cell surface glycoproteins involved in the stimulation of interleukin 1-dependent interleukin 2 production by a subline of EL4 thymoma cells. I. Functional characterization by monoclonal antibodies. J Immunol. 135(6):3944-50.
Luscher B, Rousseaux M, Lees R, MacDonald HR, Bron C. 1985. Cell surface glycoproteins involved in the stimulation of interleukin 1-dependent interleukin 2 production by a subline of EL4 thymoma cells. II. Structure, biosynthesis, and maturation. J. Immunol. 135(6):3951-7.

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Cat. 12-4321 PE Rat IgG2a Isotype Control