Product Information

Contents: Phycoerythrin (PE) anti-human CD96 (TACTILE )
Catalog Number: 12-0969
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: NK92.39
Isotype: Mouse IgG1

Staining of normal human peripheral blood cells with FITC anti-human CD19 (HIB19) (cat. 11-0199) and 0.5 μg of PE Mouse IgG1, K Iso Cntrl (cat. 12-4714) (left) or PE anti-human CD96 (NK92.39) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-0969	Phycoerythrin (PE) anti-human CD96 (TACTILE )	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The NK92.39.1 monoclonal antibody reacts with human CD96 also known as TACTILE (T cells-activated increased late expression). CD96 is a type I membrane protein of the Immunoglobulin Superfamily. CD96 promotes NK cell adhesion to cells expressing the polio virus receptor (CD155). Therefore CD96 is an additional receptor for CD155. After binding of PVR to CD96, the PVR can be transferred to the target cell and CD96 can be internalized with bound PVR. This internalization may lead to cell death of the NK cell. This phenomenon may be significant in tumor cells which express high levels of PVR. Furthermore expression of CD96 has been found in the majority of CD34(+)CD38(-) Acute Myeloid Leukemic cells.
The NK92.39.1 antibody can block binding of soluble CD155 to NK cell lines.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This NK92.39 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This NK92.39 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μl (0.5 μg) per million cells in a 100 μl total staining volume.

References

Fuchs A, Cella M, Giurisato E, Shaw AS, Colonna M. Cutting edge: CD96 (tactile) promotes NK cell-target cell adhesion by interacting with the poliovirus receptor (CD155). J Immunol. 2004 Apr 1;172(7):3994-8. (NK92.39.1, FC, FA, PubMed)

Hosen N, Park CY, Tatsumi N, Oji Y, Sugiyama H, Gramatzki M, Krensky AM, Weissman IL. CD96 is a leukemic stem cell-specific marker in human acute myeloid leukemia. Proc Natl Acad Sci U S A. 2007 Jun 26;104(26):11008-13. Epub 2007 Jun 18.

Related Products

Cat. 12-4714 Phycoerythrin (PE) Mouse IgG1, K Isotype Control