Product Information

Contents: Phycoerythrin (PE) anti-human CD95 (Fas/APO-1)
Catalog Number: 12-0959
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: DX2
Isotype: Mouse IgG1, κ
HLDA No.: VI C64

Staining of normal human peripheral blood cells with PE Mouse IgG1, K Iso Cntrl (cat. 12-4714) (open histogram) or PE anti-human CD95 (DX2) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0959	Affinity Purified anti-human CD95 (Fas/APO-1)	N/A	N/A	FA FC IHC
13-0959	Biotin anti-human CD95 (Fas/APO-1)	N/A	N/A	FC
11-0959	FITC anti-human CD95 (Fas/APO-1)	488	518	FC
12-0959	PE anti-human CD95 (Fas/APO-1)	488	575	FC
15-0959	PE-Cy5 anti-human CD95 (Fas/APO-1)	488	670	FC
17-0959	Allophycocyanin (APC) anti-human CD95 (Fas/APO-1)	633	660	FC
25-0959	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD95 (Fas/APO-1)	488	760	FC
48-0959	eFluor^TM 450 anti-human CD95 (Fas/APO-1) (Pacific Blue® Replacement)	405	450	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The DX2 monoclonal antibody reacts with human CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. DX2 does not block binding of EOS9.1, another antibody specific for human CD95.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The DX2 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The DX2 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.125 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Schlossman, S., L. Bloumsell, et al. eds. (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
Kishimoto, T., A.E.G., von dem Borne, et al. eds. (1998). Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing Inc. London.

Related Products

Cat. 14-0958  Affinity Purified anti-human CD95 (Fas/APO-1) (clone EOS9.1)
Cat. 16-0958  Functional Grade Purified anti-human CD95 (Fas/APO-1) (clone EOS9.1)
Cat. 12-4714  Phycoerythrin (PE) Mouse IgG1, K Isotype Control