Contents: Phycoerythrin (PE) anti-mouse CD95 (Fas/APO-1)
Catalog Number: 12-0951
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 15A7
Isotype: Mouse IgG1, κ
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Surface staining of mouse thymocytes with 0.5μg PE anti-mouse CD95 (15A7). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-0951 |
Affinity Purified anti-mouse CD95 (Fas/APO-1) |
N/A |
N/A |
FC
|
| 13-0951 |
Biotin anti-mouse CD95 (Fas/APO-1) |
N/A |
N/A |
FC
|
| 12-0951 |
PE anti-mouse CD95 (Fas/APO-1) |
488 |
575 |
FC
|
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific BlueŽ and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The 15A7 monoclonal antibody reacts with mouse CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, activated lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. 15A7 reacts with mouse CD95 in BALB/c strain and a bit more weakly in C57Bl/6 strain. 15A7 does not induce apoptosis of Fas-expressing cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 15A7 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The 15A7 antibody has been tested by flow cytometric analysis of BALB/c mouse splenocyte and thymocyte cell suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Yonehara, S. 1999. Effects of anti-Fas antibodies on lymphocytes and other organs: preparation of original and new monoclonal antibodies and amelioration of systemic autoimmune disease. Int Rev Immunol 18(4): 329-45.
Nishimura, Y., A. Ishii, et al. 1995. Expression and function of mouse Fas antigen on immature and mature T cells. J Immunol 154(9): 4395-403.
Related Products
Cat. 12-4714 Phycoerythrin (PE) Mouse IgG1, K Isotype Control
Mouse Cellular Antigens Chart