Product Information

Contents: Phycoerythrin (PE) anti-human CD94
Catalog Number: 12-0949
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: DX22
Isotype: Mouse IgG1, κ
HLDA No.: N/A

Staining of normal human peripheral blood cells with staining buffer (autofluorescence) or PE DX22 (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0949	Affinity Purified anti-human CD94	N/A	N/A	FC IHC
11-0949	FITC anti-human CD94	488	518	FC
12-0949	PE anti-human CD94	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The DX22 monoclonal antibody reacts with human CD94, a 70 kDa type II transmembrane glycoprotein. CD94 belongs to the C-type lectin superfamily and is present as a heterodimer with NKG2 on the surface. CD94 is expressed by NK cells, a subset of gd T cells, and NKT cells and plays an important role in adhesion and activation of NK cell lineage.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The DX22 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The DX22 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.125 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Lazetic, S., C. Chang, et al. (1996). "Human natural killer cell receptors involved in MHC class I recognition are disulfide-linked heterodimers of CD94 and NKG2 subunits." J Immunol 157(11): 4741-5.
Phillips, J. H., C. Chang, et al. (1996). "CD94 and a novel associated protein (94AP) form a NK cell receptor involved in the recognition of HLA-A, HLA-B, and HLA-C allotypes." Immunity 5(2): 163-72.
Chang, C., A. Rodriguez, et al. (1995). "Molecular characterization of human CD94: a type II membrane glycoprotein related to the C-type lectin superfamily." Eur J Immunol 25(9): 2433-7.

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