Contents: Phycoerythrin (PE) anti-mouse CD94
Catalog Number: 12-0941
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 18d3
Isotype: Rat IgG2a, κ
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|
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-0941 |
Affinity Purified anti-mouse CD94 |
N/A |
N/A |
FC
|
| 13-0941 |
Biotin anti-mouse CD94 |
N/A |
N/A |
FC
|
| 11-0941 |
FITC anti-mouse CD94 |
488 |
518 |
FC
|
| 12-0941 |
PE anti-mouse CD94 |
488 |
575 |
FC
|
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific BlueŽ and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The 18d3 monoclonal antibody reacts with mouse CD94, a 70 kDa type II transmembrane glycoprotein. CD94 belongs to the C-type lectin superfamily and is present as a heterodimer with NKG2 on the surface. CD94 is expressed by NK cells, a subset of T cells, and NKT cells and plays an important role in adhesion and activation of NK cell lineage. DBA/2J mice are naturally CD94-deficient and do not express cell-surface CD94/NKG2A receptors, even on neonatal NK cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 18d3 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The 18d3 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Vance, R. E., A. M. Jamieson, et al. (1999). "Recognition of the class Ib molecule Qa-1(b) by putative activating receptors CD94/NKG2C and CD94/NKG2E on mouse natural killer cells." J Exp Med 190(12): 1801-12.
Vance, R. E., J. R. Kraft, et al. (1998). "Mouse CD94/NKG2A is a natural killer cell receptor for the nonclassical major histocompatibility complex (MHC) class I molecule Qa-1(b)." J Exp Med 188(10): 1841-8.
Vance, R. E., D. M. Tanamachi, et al. (1997). "Cloning of a mouse homolog of CD94 extends the family of C-type lectins on murine natural killer cells." Eur J Immunol 27(12): 3236-41.
Related Products
Cat. 12-4321 PE Rat IgG2a Isotype Control
Mouse Cellular Antigens Chart