Product Information

Contents: Phycoerythrin (PE) anti-mouse CD90.2 (Thy-1.2, Thy1.2)
Catalog Number: 12-0903
Sizes: 50 ug, 100 ug, 200 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 30-H12
Isotype: Rat IgG2b, κ

Staining of C57Bl/6 splenocytes with staining buffer (autofluorescence) (open histogram) or 0.06 µg of PE 30-H12 (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0903	Affinity Purified anti-mouse CD90.2 (Thy-1.2, Thy1.2)	N/A	N/A	FA FC IH/F
16-0903	Functional Grade* Purified anti-mouse CD90.2 (Thy-1.2, Thy1.2)	N/A	N/A	FA FC
13-0903	Biotin anti-mouse CD90.2 (Thy-1.2, Thy1.2)	N/A	N/A	FC
11-0903	FITC anti-mouse CD90.2 (Thy-1.2, Thy1.2)	488	518	FC
12-0903	PE anti-mouse CD90.2 (Thy-1.2, Thy1.2)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 30-H12 monoclonal antibody reacts with mouse CD90.2, also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 30-H12. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 30-H12 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The 30-H12 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Sugai M, Kondo S, Shimizu A, Honjo T. Isolation of differentially expressed genes upon immunoglobulin class switching by a subtractive hybridization method using uracil DNA glycosylase. Nucleic Acids Res. 1998 Feb 5;26(4):911-8. (In vitro depletion)

Ledbetter, J.A. and L.A. Herzenberg (1979). "Xenogenic monoclonal antibodies to mouse lymphoid differentiation antigens." Immunol Rev 47: 63-90.

Related Products

Cat. 11-0902  FITC anti-mouse CD90.2 (Thy-1.2, Thy1.2) (clone 53-2.1)
Cat. 12-0902  PE anti-mouse CD90.2 (Thy-1.2, Thy1.2) (clone 53-2.1)
Cat. 13-0902  Biotin anti-mouse CD90.2 (Thy-1.2, Thy1.2) (clone 53-2.1)
Cat. 14-0902  Affinity Purified anti-mouse CD90.2 (Thy-1.2, Thy1.2) (clone 53-2.1)
Cat. 15-0902  PE-Cy5 anti-mouse CD90.2 (Thy-1.2, Thy1.2) (clone 53-2.1)
Cat. 17-0902  APC anti-mouse CD90.2 (Thy-1.2, Thy1.2) (clone 53-2.1)
Cat. 12-4031  Phycoerythrin (PE) Rat IgG2b Isotype Control