Print Page

Adobe PDF

Mouse Cellular Antigens Chart

Contents: Phycoerythrin (PE) anti-mouse CD86 (B7-2) Catalog Number: 12-0861 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 4°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL. Clone: PO3.1 Isotype: Rat IgG2b, κ Prices for This Product* Cat. No. Size Price Qty Action 12-0861-81 50 ug $100 12-0861-82 100 ug $195 12-0861-83 200 ug $280 *International customers: Please contact your distributor for region specific pricing.

Surface staining of LPS stimulated splenocytes with anti-mouse CD86 (PO3.1) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-0861	PE anti-mouse CD86 (B7-2, B72)	488	575	FC
14-0861	Affinity Purified anti-mouse CD86 (B7-2, B72)	N/A	N/A	FA  FC
16-0861	Functional Grade* Purified anti-mouse CD86 (B7-2, B72)	N/A	N/A	FA  FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.   Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

---

Description

---

The PO3.1 monoclonal antibody reacts with mouse CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low level on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. CD86 is also expressed by activated mouse T cells and thioglycolate-elicited peritoneal cells. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). The interaction of CD86 with its ligands plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation supports its major contribution during the primary phase of an immune response.

---

Applications Reported

---

For research use only, not for diagnostic or therapeutic use. The PO3.1 antibody has been reported for use in flow cytometric analysis.

---

Applications Tested

---

The PO3.1 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocyte suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

---

References

---

Nakajima, A., M. Azuma, et al. (1995). "Preferential dependence of autoantibody production in murine lupus on CD86 costimulatory molecule." Eur J Immunol 25(11): 3060-9.

---

Related Products

---

Cat. 11-0862    FITC anti-mouse CD86 (B7-2, B72) (clone GL1)
Cat. 12-0862    PE anti-mouse CD86 (B7-2, B72) (clone GL1)
Cat. 13-0862    Biotin anti-mouse CD86 (B7-2, B72) (clone GL1)
Cat. 14-0862    Affinity Purified anti-mouse CD86 (B7-2, B72) (clone GL1)
Cat. 15-0862    PE-Cy5 anti-mouse CD86 (B7-2, B72) (clone GL1)
Cat. 16-0862    Functional Grade Purified anti-mouse CD86 (B7-2, B72) (clone GL1)
Cat. 17-0862    APC anti-mouse CD86 (B7-2, B72) (clone GL1)
Cat. 12-4031    Phycoerythrin (PE) Rat IgG2b Isotype Control