Product Information

Contents: Phycoerythrin (PE) anti-mouse CD83
Catalog Number: 12-0831
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: Michel-17 (Michel17)
Isotype: Rat IgG1

Staining of 3-day unstimulated (left) and 3-day LPS activated (right) BALB/c splenocytes with 0.25 μg PE Rat IgG1 Iso Cntrl (cat. 12-4301) (open histogram) or 0.25 μg PE anti-mouse CD83 (Michel-17)(colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0831	Purified anti-mouse CD83	N/A	N/A	FC
16-0831	Functional Grade* Purified anti-mouse CD83	N/A	N/A	FC
13-0831	Biotin anti-mouse CD83	N/A	N/A	FC
11-0831	Fluorescein isothiocyanate (FITC) anti-mouse CD83	488	518	FC
12-0831	Phycoerythrin (PE) anti-mouse CD83	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The Michel-17 monoclonal antibody reacts with mouse CD83, a 45kDa cell surface glycoprotein and a member of the Ig superfamily. The mouse CD83 antigen is expressed predominantly on mature DC and activated lymphocytes. Cross-linking of CD83 with Michel-17 on DC or activated T cells does not induce any activation signal. CD83 plays an important role in T cell development through interaction with its ligand. CD83-Ig protein has revealed the presence of a CD83 ligand expressed mainly by B220⁺ cells in mouse spleen.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The Michel-17 (Michel17) antibody has been reported for use in flow cytometric analysis.

Applications Tested

The Michel-17 (Michel17) antibody has been tested by flow cytometric analysis of LPS activated mouse splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Wolenski M, Cramer SO, Ehrlich S, Steeg C, Grossschupff G, Tenner-Racz K, Racz P, Fleischer B, von Bonin A. 2003. Expression of CD83 in the murine immune system. Med Microbiol Immunol. 192(4):189-92.

Cramer SO, Trumpfheller C, Mehlhoop U, More S, Fleischer B, von Bonin A. 2000. Activation-induced expression of murine CD83 on T cells and identification of a specific CD83 ligand on murine B cells. Int Immunol. 12(9):1347-51.

Wolenski M, Cramer SO, Ehrlich S, Steeg C, Fleischer B, von Bonin A. 2003. Enhanced activation of CD83-positive T cells. Scand J Immunol. 58(3):306-11.

Related Products

Cat. 12-4301 PE Rat IgG1 Isotype Control