Contents: Phycoerythrin (PE) anti-human CD80 (B7-1)
Catalog Number: 12-0809
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 2D10.4
Isotype: Mouse IgG1, κ
HLDA No.: VI B-CD80.1
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-0809 |
Affinity Purified anti-human CD80 (B7-1, B71) |
N/A |
N/A |
FC
|
| 16-0809 |
Functional Grade* Purified anti-human CD80 (B7-1, B71) |
N/A |
N/A |
FA
FC
|
| 13-0809 |
Biotin anti-human CD80 (B7-1, B71) |
N/A |
N/A |
FC
|
| 11-0809 |
FITC anti-human CD80 (B7-1, B71) |
488 |
518 |
FC
|
| 12-0809 |
PE anti-human CD80 (B7-1, B71) |
488 |
575 |
FC
|
| 15-0809 |
Phycoerythrin-Cy5 (PE-Cy5) anti-human CD80 (B7-1, B71) |
488 |
670 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily. CD80 is expressed by activated B cells, macrophages and dendritic cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 2D10.4 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The 2D10.4 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).
References
Bashuda, H., Kimikawa, M., Seino, K., Kato, Y., Ono, F., Shimizu, A., Yagita, H., Teraoka, S., and K. Okumura. 2005. Renal allograft rejection is prevented by adoptive transfer of anergic T cells in nonhuman primates. J Clin Invest.115:1896-1902. (2D10.4, FA,
PubMed)
Kishimoto, T., A.E.G., von dem Borne, et al. eds. 1998 .Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing Inc. London.
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Human Cellular Antigens Chart