Product Information

Contents: Phycoerythrin (PE) anti-rat CD80 (B7-1)
Catalog Number: 12-0800
Sizes: 50 ug, 100 ug, 200 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 3H5
Isotype: Mouse IgG1, κ

Surface staining of LPS stimulated rat splenocytes with 0.25 μg PE anti-rat CD80 (3H5). Autofluorescence is indicated by open histogram. Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0800	Affinity Purified anti-rat CD80 (B7-1, B71)	N/A	N/A	FA FC IH/F IP
16-0800	Functional Grade* Purified anti-rat CD80 (B7-1, B71)	N/A	N/A	FA FC
13-0800	Biotin anti-rat CD80 (B7-1, B71)	N/A	N/A	FC
12-0800	PE anti-rat CD80 (B7-1, B71)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 3H5 monoclonal antibody reacts with rat CD80 (B7-1), an ~70 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively. 3H5 blocks binding of CD80 to its ligands.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 3H5 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The 3H5 antibody has been tested by flow cytometric analysis of activated rat splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Maeda, K., T. Sato, et al. (1997). "Characterization of rat CD80 and CD86 by molecular cloning and mAb." Int Immunol 9(7): 993-1000.
Damoiseaux, J. G., H. Yagita, et al. (1998). "Costimulatory molecules CD80 and CD86 in the rat; tissue distribution and expression by antigen-presenting cells." J Leukoc Biol 64(6): 803-9.

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