Contents: Phycoerythrin (PE) anti-rat CD80 (B7-1)
Catalog Number: 12-0800
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 3H5
Isotype: Mouse IgG1, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
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Surface staining of LPS stimulated rat splenocytes with 0.25 μg PE anti-rat CD80 (3H5). Autofluorescence is indicated by open histogram. Total viable cells were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-0800 |
Affinity Purified anti-rat CD80 (B7-1, B71) |
N/A |
N/A |
FA
FC
IH/F
IP
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| 16-0800 |
Functional Grade* Purified anti-rat CD80 (B7-1, B71) |
N/A |
N/A |
FA
FC
|
| 13-0800 |
Biotin anti-rat CD80 (B7-1, B71) |
N/A |
N/A |
FC
|
| 12-0800 |
PE anti-rat CD80 (B7-1, B71) |
488 |
575 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The 3H5 monoclonal antibody reacts with rat CD80 (B7-1), an ~70 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively. 3H5 blocks binding of CD80 to its ligands.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 3H5 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The 3H5 antibody has been tested by flow cytometric analysis of activated rat splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Maeda, K., T. Sato, et al. (1997). "Characterization of rat CD80 and CD86 by molecular cloning and mAb."
Int Immunol 9(7): 993-1000.
Damoiseaux, J. G., H. Yagita, et al. (1998). "Costimulatory molecules CD80 and CD86 in the rat; tissue distribution and expression by antigen-presenting cells."
J Leukoc Biol 64(6): 803-9.
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