Product Information

Contents: Phycoerythrin (PE) anti-human CD71 (transferrin receptor)
Catalog Number: 12-0719
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: OKT9 (OKT-9)
Isotype: Mouse IgG1

Staining of 3-day PHA activated normal human PBMCs with PE Mouse IgG1, K Iso Cntrl (cat. 12-4714) (open histogram) or PE anti-human CD71 (OKT9) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0719	Affinity Purified anti-human CD71 (transferrin receptor)	N/A	N/A	FC
16-0719	Functional Grade* Purified anti-human CD71 (transferrin receptor)	N/A	N/A	FC
13-0719	Biotin anti-human CD71 (transferrin receptor)	N/A	N/A	FC
11-0719	Fluorescein isothiocyanate (FITC) anti-human CD71 (transferrin receptor)	488	518	FC
12-0719	Phycoerythrin (PE) anti-human CD71 (transferrin receptor)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The OKT9 monoclonal antibody reacts with human CD71, a 170-180 kDa type II transmembrane protein. CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth. CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes. Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This OKT9 (OKT-9) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This OKT9 (OKT-9) antibody has been pre-titrated and tested by flow cytometric analysis of PHA-stimulated human peripheral blood mononuclear cells. This can be used at 20 μl (.06 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Sutherland R, Delia D, Schneider C, Newman R, Kemshead J, Greaves M. 1981. Ubiquitous cell-surface glycoprotein on tumor cells is proliferation-associated receptor for transferrin. Proc Natl Acad Sci U S A. 78(7): 4515–4519.

Salcedo TW, Fleit HB. 1991. Plasma membrane and intracellular pools of transferrin receptors decline during in vitro cultivation of U937 cells. Cell Prolif. 24(4):383-401.

Wenning LA, Murphy RM. 1999. Coupled cellular trafficking and diffusional limitations in delivery of immunotoxins to multicell tumor spheroids. Biotechnol Bioeng. 62(5):562-75.

Related Products

Cat. 11-0717  Fluorescein isothiocyanate (FITC) anti-human CD71 (Transferrin Receptor) (clone T56/14)
Cat. 12-0717  Phycoerythrin (PE) anti-human CD71 (Transferrin Receptor) (clone T56/14)
Cat. 14-0717  Affinity Purified anti-human CD71 (Transferrin Receptor) (clone T56/14)
Cat. 12-4714  Phycoerythrin (PE) Mouse IgG1, K Isotype Control