PE anti-mouse CD70 antibody: r-phycoerythin (r-PE) conjugate

Contents: Phycoerythrin (PE) anti-mouse CD70
Catalog Number: 12-0701
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: FR70
Isotype: Rat IgG2b, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
12-0701-81	50 ug	$150
12-0701-82	100 ug	$205
12-0701-83	200 ug	$350

*International customers: Please contact your distributor for region specific pricing.

Surface staining of LPS + anti-mouse CD40 (cat. 16-0401) stimulated mouse splenocytes with 0.25 μg PE anti-mouse CD70 (FR70). Autofluorescence is shown via open histogram. Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0701	Affinity Purified anti-mouse CD70	N/A	N/A	FA FC IP
16-0701	Functional Grade* Purified anti-mouse CD70	N/A	N/A	FC
13-0701	Biotin anti-mouse CD70	N/A	N/A	FC
12-0701	PE anti-mouse CD70	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Description

The FR70 monoclonal antibody reacts with mouse CD70, a 30-33 kDa type II transmembrane glycoprotein. CD70 is a member of the TNF family and is expressed by activated mouse T and B lymphocytes. Interaction of CD70 with CD27, its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.

Applications Reported

For research use only, not for diagnostic or therapeutic use.

Applications Tested

The FR70 antibody has been tested by flow cytometric analysis of mouse LPS-activated splenocyte suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Oshima, H., H. Nakano, et al. (1998). "Characterization of murine CD70 by molecular cloning and mAb." Int Immunol 10(4): 517-26.
Akiba, H., H. Oshima, et al. (1999). "CD28-independent costimulation of T cells by OX40 ligand and CD70 on activated B cells." J Immunol 162(12): 7058-66.

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