Product Information

Contents: Phycoerythrin (PE) anti-rat Macrophages
Catalog Number: 12-0660
Sizes: 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HIS36
Isotype: Mouse IgG2a, κ

Surface staining of rat thioglycolate induced PEC with 0.25 μg PE anti-rat Macrophages (HIS36). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0660	Affinity Purified anti-rat Macrophages	N/A	N/A	FC IH/F
12-0660	PE anti-rat Macrophages	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The HIS36 monoclonal antibody reacts with a molecule expressed on the surface of rat macrophages. The antigen recognized by HIS36 is an ED2-like antigen expressed by thioglycolate-elicited peritoneal cells and tissue macrophages. This antibody is used as a marker for rat tissue macrophages.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HIS36 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The HIS36 antibody has been tested by flow cytometric analysis of rat spleen and peritoneal cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Van Goor, H., G. Harms, et al. 1988. Immunohistochemical antigen demonstration in plastic-embedded lymphoid tissue. J Histochem Cytochem 36(1): 115-20.
Shirasawa, B., K. Hamano, et al. 2000. Contribution of proliferating leukocytes to phenotypic change in smooth muscle cells during the development of coronary arteriosclerosis in transplanted hearts. Eur Surg Res 32(1): 30-8.

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Cat. 12-4724 Phycoerythrin (PE) Mouse IgG2a, K Isotype Control