Product Information

Contents: Phycoerythrin (PE) anti-human CD62E (E-selectin)
Catalog Number: 12-0627
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: P2H3
Isotype: Mouse IgG1

Staining of TNFα-activated Human Umbilical Vein Endothelial Cells (HUVEC) with PE Mouse IgG1, K Iso Cntrl (cat. 12-4714) (open histogram) or PE anti-human CD62E (P2H3) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0627	Affinity Purified anti-human CD62E (E-selectin)	N/A	N/A	FA FC IHC IP
12-0627	Phycoerythrin (PE) anti-human CD62E (E-selectin)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The P2H3 monoclonal antibody reacts with human CD62E, a 97-115 kDa member of the selectin family. CD62E, also known as E-selectin or endothelial-leukocyte adhesion molecule-1 (ELAM-1) is an adhesion molecule expressed by endothelial cells upon stimulation with cytokines including TNFα and IL-1β. Induced expression of CD62E during inflammatory conditions is thought to mediate leukocyte rolling including the initial interaction of neutrophils with endothelium.

The P2H3 monoclonal antibody also inhibits cellular adhesion to cytokine-activated endothelial cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This P2H3 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This P2H3 antibody has been pre-titrated and tested by flow cytometric analysis of TNFα-actiavted Human Umbilical Vein Endothelial Cells (HUVEC). This can be used at 20 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Bevilacqua MP, Stengelin S, Gimbrone MA Jr, Seed B. Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins. Science. 1989 Mar 3;243(4895):1160-5.

Graber N, Gopal TV, Wilson D, Beall LD, Polte T, Newman W. T cells bind to cytokine-activated endothelial cells via a novel, inducible sialoglycoprotein and endothelial leukocyte adhesion molecule-1. J Immunol. 1990 Aug 1;145(3):819-30.

Phillips ML, Nudelman E, Gaeta FC, Perez M, Singhal AK, Hakomori S, Paulson JC. ELAM-1 mediates cell adhesion by recognition of a carbohydrate ligand, sialyl-Lex. Science. 1990 Nov 23;250(4984):1130-2.

Related Products

Cat. 12-4714 Phycoerythrin (PE) Mouse IgG1, K Isotype Control