Product Information

Contents: Phycoerythrin (PE) anti-human CD61 (integrin β3)
Catalog Number: 12-0619
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: VI-PL2
Isotype: Mouse IgG1, κ

Staining of normal human peripheral blood platelets with FITC anti-human CD42b (HIP1) (cat. 11-0429) and PE Mouse IgG1, K Iso Cntrl (cat. 12-4714) (left) or PE anti-human CD61 (VI-PL2) (right). CD42b (-) events represent cell debris.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0619	Affinity Purified anti-human CD61 (integrin beta 3)	N/A	N/A	FA FC IH/F WB
11-0619	Fluorescein isothiocyanate (FITC) anti-human CD61 (integrin beta 3)	488	518	FC
12-0619	Phycoerythrin (PE) anti-human CD61 (integrin beta 3)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The VI-PL2 monoclonal antibody reacts with human CD61, also known as integrin β3 and GPIIIa. CD61 is a 90-110 kDa member of the /beta integrin family expressed by a wide variety of cells, including leukocytes, platelets, endothelial and smooth muscle cells. CD61 binds non-covalently with the α integrins CD41 and CD51, to form the αIIbβ3 (CD41/CD61) and αvβ3 (CD51/CD61) complexes. Theseαβ heterodimers are capable of mediating a variety of cellular responses including adhesion, trafficking, proliferaton and differentiation. The CD41/CD61 integrin has been shown to be involved in platelet aggregation, and binds to fibrinogen, von Willebrand Factor (vWF) and fibronectin. The CD51/CD61 integrin binds to matrix proteins including vitronectin, fibronectin, vWF and fibrinogen, and has been shown to have a strong role in modulating the migration and survival of angiogenic endothelial cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This VI-PL2 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This VI-PL2 antibody has been tested by flow cytometric analysis of human peripheral blood platelets. This can be used at 20 μl (0.06 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Barrett L, Dai C, Gamberg J, Gallant M, Grant M. Circulating CD14-CD36+ peripheral blood mononuclear cells constitutively produce interleukin-10. J Leukoc Biol. 2007 Jul;82(1):152-60. (VI-PL2, FC, PubMed)

Roberts MS, Woods AJ, Dale TC, Van Der Sluijs P, Norman JC. Protein kinase B/Akt acts via glycogen synthase kinase 3 to regulate recycling of alpha v beta 3 and alpha 5 beta 1 integrins. Mol Cell Biol. 2004 Feb;24(4):1505-15. (VI-PL2, FA, PubMed)

Ciarlet M, Crawford SE, Cheng E, Blutt SE, Rice DA, Bergelson JM, Estes MK. VLA-2 (alpha2beta1) integrin promotes rotavirus entry into cells but is not necessary for rotavirus attachment. J Virol. 2002 Feb;76(3):1109-23. (VI-PL2, FA, PubMed)

Related Products

Cat. 12-0611 PE anti-mouse CD61 (Integrin beta 3) (clone 2C9.G3)
Cat. 12-4714 Phycoerythrin (PE) Mouse IgG1, K Isotype Control