Product Information

Contents: Phycoerythrin (PE) anti-mouse CD61
Catalog Number: 12-0611
Sizes: 50 ug, 100 ug, 200 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 2C9.G3
Isotype: Armenian Hamster IgG

Surface staining of mouse bone marrow with FITC (left) anti-mouse CD61 (2C9.G30) and 0.25 μg PE (right) anti-mouse CD61 (2C9.G3). Appropriate isotype controls were used (open histogram). Cells in the myeloid population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0611	Affinity Purified anti-mouse CD61 (Integrin beta 3)	N/A	N/A	FA FC IH/F
16-0611	Functional Grade* Purified anti-mouse CD61 (Integrin beta 3)	N/A	N/A	FA FC
13-0611	Biotin anti-mouse CD61 (Integrin beta 3)	N/A	N/A	FC
11-0611	FITC anti-mouse CD61 (Integrin beta 3)	488	518	FC
12-0611	PE anti-mouse CD61 (Integrin beta 3)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 2C9.G3 (HMb3-1) monoclonal antibody reacts with mouse CD61, also known as the integrin β₃. CD61 is expressed by activated T cells, granulocytes, and platelet. CD61 associates non-covalently with two integrin α subunits; α_V (CD51) to form Vitronectin Receptor and α_Iib (CD41) to form gpIIb/IIIa. These heterodimeric complexes are responsible for adhesion to extracellular matrix components including fibrinogen, fibronectin, fibronectin, vitronectin, thrombospondin and von Willebrand factor.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 2C9.G3 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The 2C9.G3 antibody has been tested by flow cytometric analysis of mouse splenocyte and bone marrow cell suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Yasuda M, Hasunuma Y, Adachi H, Sekine C, Sakanishi T, Hashimoto H, Ra C, Yagita H, Okumura K. 1995. Expression and function of fibronectin binding integrins on rat mast cells. Int Immunol. 7:251-8.

Nohara K, Pan X, et al. 2005. Constitutively active aryl hydrocarbon receptor expressed specifically in T-lineage cells causes thymus involution and suppresses the immunization-induced increase in splenocytes. J Immunol. 174(5):2770-7. (FC, PubMed)

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