Product Information

Contents: Phycoerythrin (PE) anti-human CD59 (Protectin)
Catalog Number: 12-0597
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: MEM-43 (MEM43)
Isotype: Mouse IgG2a
HLDA No.: V

Staining of normal human peripheral blood cells with PE Mouse IgG2a, K Iso Cntrl (cat. 12-4724) (open histogram) or PE anti-human CD59 (MEM43) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0597	Fluorescein isothiocyanate (FITC) anti-human CD59 (Protectin)	488	518	FC
12-0597	Phycoerythrin (PE) anti-human CD59 (Protectin)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The MEM-43 monoclonal antibody reacts with human CD59, also known as protectin, membrane inhibitor of reactive lysis (MIRL), and membrane-attack-complex-inhibitory factor (MACIF). CD59 is an approximately 19 kDa GPI-linked glycoprotein expressed by hematopoietic and non-hematopoietic cells. Through binding complement components C8 and C9, CD59 inhibits the assembly of the membrane attack complex and the cytolytic activity by complement. CD59 also interacts with CD2 and has a role in T cell activation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MEM-43 (MEM43) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This MEM-43 (MEM43) antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 5 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Schlossman, S., L. Bloumsell, et al. eds. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 12-4724 Phycoerythrin (PE) Mouse IgG2a, K Isotype Control