Product Information

Contents: Phycoerythrin (PE) anti-human CD55
Catalog Number: 12-0559
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 143-30
Isotype: Mouse IgG1, κ
HLDA No.: IV NL-7

Staining of normal human peripheral blood cells with 1 μg of PE Mouse IgG1, K Iso Cntrl (cat. 12-4714) (open histogram) or PE anti-human CD55 (143-30) (colored histogram). Cells in the lymphocyte and monocyte gates were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0559	Affinity Purified anti-human CD55	N/A	N/A	FC
13-0559	Biotin anti-human CD55	N/A	N/A	FC
11-0559	FITC anti-human CD55	488	518	FC
12-0559	PE anti-human CD55	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 143-30 monoclonal antibody reacts with human CD55, the 64-73 kDa molecule also known as DAF (Decay-Accelerating Factor). CD55 is a GPI-anchored single chain glycoprotein expressed on all cells in contact with serum including all hematopoietic cells and vascular endothelium; a soluble form of CD55 is also present in plasma and body fluids. CD55 is thought to play a role in the protection of cells from the damage by autologous complement via prevention of the amplification steps of the complement cascade. Antibodies against CD55 are mitogenic in the presence of phorbol esters.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 143-30 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 143-30 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.25 μg) per million cells in a 100 μl total staining volume.

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 12-4714 Phycoerythrin (PE) Mouse IgG1, K Isotype Control