Product Information

Contents: Phycoerythrin (PE) anti-mouse CD51 (Integrin α_V)
Catalog Number: 12-0512
Sizes: 50 ug, 100 ug, 200 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: RMV-7
Isotype: Rat IgG1, κ

Staining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.25 µg PE anti-mouse CD51 (RMV-7) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0512	Affinity Purified anti-mouse CD51 (Integrin alpha v)	N/A	N/A	FA FC IP WB
16-0512	Functional Grade* Purified anti-mouse CD51 (Integrin alpha v)	N/A	N/A	FA FC
13-0512	Biotin anti-mouse CD51 (Integrin alpha v)	N/A	N/A	FC
12-0512	PE anti-mouse CD51 (Integrin alpha v)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Description

The RMV-7 monoclonal antibody reacts with the mouse CD51 molecule, the integrin alpha v chain. This approximately 120 kDa surface molecule non-covalently associates with the β subunits of the integrin family including β₃ (CD61), β₁ (CD29), β₅ and β₆ to form receptors for extracellular matrix components. Heterodimers of CD51/CD61 are expressed by platelets, T cells and granulocytes and mediate adhesion to fibrinogen, fibronectin, vitronectin and thrombospondin.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The RMV-7 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The RMV-7 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Tsujimura, K., T. Takahashi, et al. (1998). "Two types of anti-TL (thymus leukemia) CTL clones with distinct target specificities: differences in cytotoxic mechanisms and accessory molecule requirements." J Immunol 160(11): 5253-61.
Takahashi, K., T. Nakamura, et al. (1991). "Antigen-independent T cell activation mediated by a very late activation antigen-like extracellular matrix receptor." Eur J Immunol 21(6): 1559-62.
Takahashi, K., T. Nakamura, M. Koyanagi, K. Kato, Y. Hashimoto, H. Yagita, and K. Okumura. 1990. A murine very late activation antigen-like extracellular matrix receptor involved in CD2- and lymphocyte function-associated antigen-1-independent killer-target cell interaction. J. Immunol. 145:4371-4379

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