Product Information

Contents: Phycoerythrin (PE) anti-human Integrin Beta 5
Catalog Number: 12-0497
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: KN52
Isotype: Mouse IgG1, κ

Staining of CHO cells with PE Mouse IgG1, K Iso Cntrl (cat. 12-4714) (open histogram) or PE anti-human Integrin beta 5 (KN52) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0497	Affinity Purified anti-human/ mouse/ hamster Integrin Beta 5	N/A	N/A	FA FC
16-0497	Functional Grade* Purified anti-human/ mouse/ hamster Integrin Beta 5	N/A	N/A	FA FC
11-0497	Fluorescein isothiocyanate (FITC) anti-human/ mouse/ hamster Integrin Beta 5	488	518	FC
12-0497	Phycoerythrin (PE) anti-human/ mouse/ hamster Integrin Beta 5	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The KN52 antibody reacts with human integrin beta 5, an approximately 100,000 Mr (reduced) and 95,000 Mr (nonreduced) a member of the integrin family. Only a single alpha chain, the alpha v subunit, associates with the integrin beta 5 to form the vitronectin receptor complex. Integrin alpha v/beta 5 complex also binds to the basic domain of Tat (he sequence RKKRRQRRR). The integrin beta 5 is found on carcinoma cell lines, hepatoma and fibroblast cell lines, and is absent from lymphoblastoid cells and platelets.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This KN52 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This KN52 antibody has been pre-titrated and tested by flow cytometric analysis of Chinese Hamster Ovary (CHO), A549 and normal human peripheral blood cells. This can be used at 20 μl (0.06 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Lai CF, Feng X, Nishimura R, Teitelbaum SL, Avioli LV, Ross FP, Cheng SL. Transforming growth factor-beta up-regulates the beta 5 integrin subunit expression via Sp1 and Smad signaling. J Biol Chem. 2000 Nov 17;275(46):36400-6.

Sugiyama M, Speight PM, Prime SS, Watt FM. Comparison of integrin expression and terminal differentiation capacity in cell lines derived from oral squamous cell carcinomas. Carcinogenesis. 1993 Oct;14(10):2171-6.

Related Products

Cat. 12-4714 Phycoerythrin (PE) Mouse IgG1, K Isotype Control