Product Information

Contents: Phycoerythrin (PE) anti-human/mouse CD49f (Integrin alpha 6)
Catalog Number: 12-0495
Sizes: 50 ug, 100 ug, 200 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioGoH3 (GoH3)
Isotype: Rat IgG2a, κ

Staining of normal human peripheral blood cells with 0.06 μg of PE Rat IgG2a Iso Cntrl (cat. 12-4321) (open histogram) or 0.06 μg of PE anti-hu/mo CD49f (eBioGoH3) (colored histogram). Cells in the lymphocyte (left) and granulocyte (right) gates were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0495	Affinity Purified anti-human/mouse CD49f (Integrin alpha 6)	N/A	N/A	FC IH/F IP
13-0495	Biotin anti-human/mouse CD49f (Integrin alpha 6)	N/A	N/A	FC IH/F
12-0495	Phycoerythrin (PE) anti-human/mouse CD49f (Integrin alpha 6)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBioGoH3 monoclonal antibody reacts with mouse and human CD49f, also known as integrin alpha 6, very late activation antigen 6 (VLA-6 alpha chain), and platelet gpIc. CD49f is a 120 kD transmembrane protein. CD49f associates with CD29, the integrin beta 1 chain, to form the VLA-6 complex; CD49f also associates with CD104, the integrin beta 4 chain, to form the alpha 6 beta 4 complex. CD49f is expressed primarily on T cells, monocytes, platelets, epithelial and endothelial cells. CD49f expression has also been found on germinal center B cells. The eBioGoH3 antibody is cross-reactive to integrin alpha 6 on human, mouse and bovine cells. This antibody has also been reported to have functional activity in blocking the binding of integrin alpha 6 to laminin.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioGoH3 (GoH3) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBioGoH3 (GoH3) antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.125 µg per 100 µl blood (or per 1 million cells in 100 µl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Sonnenberg A, Daams H, Van der Valk MA, Hilkens J, Hilgers J. Development of mouse mammary gland: identification of stages in differentiation of luminal and myoepithelial cells using monoclonal antibodies and polyvalent antiserum against keratin. J Histochem Cytochem. 1986 Aug;34(8):1037-46. (GoH3, FC, IH/F PubMed)

Aumailley M, Timpl R, Sonnenberg A. Antibody to integrin alpha 6 subunit specifically inhibits cell-binding to laminin fragment 8. Exp Cell Res. 1990 May;188(1):55-60. (GoH3, FA, PubMed)

Ambrose HE, Wagner SD. Alpha6-integrin is expressed on germinal centre B cells and modifies growth of a B-cell line. Immunology. 2004 Apr;111(4):400-6. (GoH3, FC, PubMed)

Related Products

Cat. 12-0291  Phycoerythrin (PE) anti-mouse CD29 (Integrin b1 chain, a4b1) (clone eBioHMb1-1 (HMb1-1))
Cat. 14-0291  Affinity Purified anti-mouse CD29 (Integrin b1 chain, a4b1) (clone eBioHMb1-1 (HMb1-1))
Cat. 11-0297  Fluorescein isothiocyanate (FITC) anti-human CD29 (Integrin beta 1) (clone MEM-101A)
Cat. 12-0297  Phycoerythrin (PE) anti-human CD29 (Integrin beta 1) (clone MEM-101A)
Cat. 14-0297  Purified anti-human CD29 (Integrin beta 1) (clone MEM-101A)
Cat. 15-0297  Phycoerythrin-Cy5 (PE-Cy5) anti-human CD29 (Integrin beta 1) (clone MEM-101A)
Cat. 12-4321  PE Rat IgG2a Isotype Control