Contents: Phycoerythrin (PE) anti-mouse/rat CD49e (Integrin α5 chain, VLA-5)
Catalog Number: 12-0493
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioHMa5-1 (HMa5-1)
Isotype: Armenian Hamster IgG
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Staining of C57Bl/6 bone marrow cells with FITC anti-mo/hu B220 (RA3-6B2) (cat. 11-0452) and 0.25 μg of PE Armenian Hamster IgG Iso Cntrl (cat. 12-4888) (left) or 0.25 μg of PE anti-mouse/rat CD49e/CD29 (Integrin α5 chain, VLA-5) (right). Cells in the large scatter population were used for analysis.
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Description
The eBioHMα5-1 monoclonal antibody reacts with mouse and rat CD49e, also known as integrin α5 or VLA-5A. CD49e is a member of the integrin α chain family, and binds with CD29 (integrin β1) to form the receptor VLA-5, which binds the ligands fibronectin and fibrinogen. CD49e is a type I, single-pass membrane protein, with a molecular weight of 135 kDa, and is expressed on multiple cell types including thymocytes, mast cells, activated T cells and splenic B cells. In addition to its role in adhesion, VLA-5 contributes to T-cell co-stimulation. The eBioHMα5-1 monoclonal antibody has been demonstrated to interfere with VLA-5-mediated cell adhesion, and with the degranulation of IgE-sensitized rat RBL cells stimulated on fibronectin-coated plates. Furthermore, HMα5-1 injected into mice subcutaneously, along with anti-CD49d and anti-CD61, is able to inhibit passive cutaneous anaphylaxis.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBioHMa5-1 (HMa5-1, HMa5-1) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBioHMa5-1 (HMa5-1, HMa5-1) antibody has been tested by flow cytometric analysis of mouse bone marrow cell suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Noto K, Kato K, Okumura K, Yagita H. Identification and functional characterization of mouse CD29 with a mAb. Int Immunol. 1995 May;7(5):835-42.
Argraves WS, Suzuki S, Arai H, Thompson K, Pierschbacher MD, Ruoslahti E.
Amino acid sequence of the human fibronectin receptor.
J Cell Biol. 1987 Sep;105(3):1183-90.
Birkenmeier TM, McQuillan JJ, Boedeker ED, Argraves WS, Ruoslahti E, Dean DC.
The alpha 5 beta 1 fibronectin receptor. Characterization of the alpha 5 gene promoter.
J Biol Chem. 1991 Oct 25;266(30):20544-9.
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