PE anti-mouse CD49d (Integrin alpha 4) antibody: r-phycoerythin (r-PE) conjugate

Contents: Phycoerythrin (PE) anti-mouse CD49d (Integrin α₄)
Catalog Number: 12-0492
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: R1-2
Isotype: Rat IgG2b, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
12-0492-81	50 ug	$100
12-0492-82	100 ug	$195
12-0492-83	200 ug	$280

*International customers: Please contact your distributor for region specific pricing.

Staining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.5 µg PE anti-mouse CD49d (R1-2) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0492	FITC anti-mouse CD49d (Integrin alpha 4)	488	518	FC
12-0492	PE anti-mouse CD49d (Integrin alpha 4)	488	575	FC
13-0492	Biotin anti-mouse CD49d (Integrin alpha 4)	N/A	N/A	FC
14-0492	Affinity Purified anti-mouse CD49d (Integrin alpha 4)	N/A	N/A	FA FC IHC IP
16-0492	Functional Grade* Purified anti-mouse CD49d (Integrin alpha 4)	N/A	N/A	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The R1-2 monoclonal antibody reacts with mouse CD49d, the 150 kDa integrin α₄ subunit. The complex of CD49d non-covalently associated with integrin β₁ (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106). This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils. CD49d also associates with integrin b7 and binds to the Mucosal vascular ADdressin (MAdCAM-1).

Applications Reported

For research use only, not for diagnostic or therapeutic use. The R1-2 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The R1-2 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Holzmann, B., B. W. McIntyre, et al. (1989). "Identification of a murine Peyer's patch--specific lymphocyte homing receptor as an integrin molecule with an alpha chain homologous to human VLA-4 alpha." Cell 56(1): 37-46.
Neuhaus, H., M. C. Hu, et al. (1991). "Cloning and expression of cDNAs for the alpha subunit of the murine lymphocyte-Peyer's patch adhesion molecule." J Cell Biol 115(4): 1149-58.

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