Product Information

Contents: Phycoerythrin (PE) anti-human CD48 (SLAM family)
Catalog Number: 12-0489
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio156-4H9 (156-4H9)
Isotype: Mouse IgG1

Staining of normal human peripheral blood mononuclear cells with APC anti-human CD3 (UCHT1) (cat. 17-0038) and PE Mouse IgG1, K Iso Cntrl (cat. 12-4714) (left) or PE anti-human CD48 (156-4H9) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0489	Affinity Purified anti-human CD48 (SLAM family)	N/A	N/A	FC
16-0489	Functional Grade* Purified anti-human CD48 (SLAM family)	N/A	N/A	FA FC
12-0489	Phycoerythrin (PE) anti-human CD48 (SLAM family)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBio156-4H9 monoclonal antibody reacts with the human CD48 antigen, also known as B cell activation marker 1, SLAM family member 2, Blast-1 (human), and OX-45 (rat). CD48, a member of the SLAM family and Ig superfamily, is a 45 kDa GPI-linked glycoprotein expressed on the majority of hematopoietic cells. CD2 is the low affinity ligand for CD48. Recent publications have reported differential expression in mice of members of the SLAM family including CD48, CD150, and CD244 among functionally distinct bone marrow hematopoietic progenitors providing a useful tool for prediction of the primitiveness of hematopoietic progenitors. Expression of CD48 in hematopoietic cells is found on many leukocytes with little to none on granulocytes and absent on platelets and erythrocytes. Expression increases on B cells with activation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio156-4H9 (156-4H9) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBio156-4H9 (156-4H9) antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood cells. This can be used at 20 μl (0.25 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Yokoyama S, Staunton D, Fisher R, Amiot M, Fortin JJ, Thorley-Lawson DA. Expression of the Blast-1 activation/adhesion molecule and its identification as CD48. J Immunol. 1991 Apr 1;146(7):2192-200.

Gonzalez-Alvaro I, Dominguez-Jimenez C, Ortiz AM, Nunez-Gonzalez V, Roda-Navarro P, Fernandez-Ruiz E, Sancho D, Sanchez-Madrid F. Interleukin-15 and interferon-gamma participate in the cross-talk between natural killer and monocytic cells required for tumour necrosis factor production. Arthritis Res Ther. 2006;8(4):R88. (156-4H9, FC, PubMed)

Stonehouse TJ, Woodhead VE, Herridge PS, Ashrafian H, George M, Chain BM, Katz DR. Molecular characterization of U937-dependent T-cell co-stimulation. Immunology. 1999 Jan;96(1):35-47. (156-4H9, FC, PubMed)

Related Products

Cat. 12-0481 Phycoerythrin (PE) anti-mouse CD48 (BCM1) (clone HM48-1)
Cat. 12-4714 Phycoerythrin (PE) Mouse IgG1, K Isotype Control