Contents: Phycoerythrin (PE) anti-rat CD45R
Catalog Number: 12-0460
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HIS24
Isotype: Mouse IgG2b, κ
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Surface staining of rat splenocytes with anti-rat CD45R (HIS24) FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
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Description
The HIS24 monoclonal antibody reacts with an isoform of CD45 expressed by rat B cells. The antigen recognized by HIS24 is found on B cells from the early stage of B cell development in the bone marrow to mature peripheral B cells, but not on plasma cells. HIS24 recognizes a 205 kDa protein by immunoblotting. CD45, also known as Leukocyte Common Antigen (LCA), has tyrosine phosphatase enzymatic activity in its cytoplasmic portion and plays an important role in activation of lymphocytes.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The HIS24 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This HIS24 antibody has been tested by flow cytometric analysis of rat splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Kroese, F. G., D. Opstelten, et al. 1985. Monoclonal antibodies to rat B lymphocyte (sub-) populations. Adv Exp Med Biol 186: 81-9.
Kroese, F. G., A. S. Wubbena, et al. 1987. B lymphocyte differentiation in the rat: production and characterization of monoclonal antibodies to B lineage-associated antigens. Eur J Immunol 17(7): 921-8.
Kroese, F. G., E. C. Butcher, et al. 1990. The rat B cell system: the anatomical localization of flow cytometry-defined B cell subpopulations. Eur J Immunol 20(7): 1527-34.
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