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Product Information

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Contents: Phycoerythrin (PE) anti-human CD45RA Catalog Number: 12-0458 Sizes: 25 tests, 100 tests Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3, 0.2% BSA Storage Conditions: Store at 4°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL. Clone: HI100 Isotype: Mouse IgG2b, κ HLDA No.: IV N906

Surface staining of normal human peripheral blood cells with anti-human CD45RA (HI100) FITC. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0458	Affinity Purified anti-human CD45RA	N/A	N/A	FC  IHC
13-0458	Biotin anti-human CD45RA	N/A	N/A	FC
11-0458	FITC anti-human CD45RA	488	518	FC
12-0458	PE anti-human CD45RA	488	575	FC
15-0458	PE-Cy5 anti-human CD45RA	488	670	FC
17-0458	Allophycocyanin (APC) anti-human CD45RA	633	660	FC
25-0458	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD45RA	488	760	FC
57-0458	Pacific Blue® anti-human CD45RA	402	421	FC
45-0458	PerCP-Cy5.5 anti-human CD45RA	488	690	FC
93-0458	eFluorTM 605NC anti-human CD45RA	350-500	605	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

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Description

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The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4⁺ peripheral T lymphocytes, CD8⁺ peripheral T lymphocytes, and B cells. The CD45RA⁺ T cell populations are mainly naive/virgin allowing the use of HI100 mAb as a phenotypic marker to discriminate T cell subsets.

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. The HI100 antibody has been reported for use in flow cytometric analysis.

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Applications Tested

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The HI100 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

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References

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Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

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Related Products

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Cat. 12-4732    PE Mouse IgG2b Isotype Control
Cat. 11-9979    FITC anti-human CD45RA (clone JS-83)
Cat. 12-9979    PE anti-human CD45RA (clone JS-83)
Cat. 13-9979    Biotin anti-human CD45RA (clone JS-83)
Cat. 14-9979    Affinity Purified anti-human CD45RA (clone JS-83)
Cat. 15-9979    PE-Cy5 anti-human CD45RA (clone JS-83)