Product Information
Contents: Phycoerythrin (PE) anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)
Catalog Number: 12-0451
Sizes: 50 ug, 100 ug, 200 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 30-F11
Isotype: Rat IgG2b, κ
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Surface staining of mouse splenocytes with FITC (left) or 0.015 μg PE (right) anti-mouse CD45 (30-F11) . Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-0451 |
Affinity Purified anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) |
N/A |
N/A |
FA
FC
IHC
IP
|
| 16-0451 |
Functional Grade* Purified anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) |
N/A |
N/A |
FA
FC
IHC
IP
|
| 13-0451 |
Biotin anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) |
N/A |
N/A |
FC
|
| 11-0451 |
FITC anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) |
488 |
518 |
FC
|
| 12-0451 |
PE anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) |
488 |
575 |
FC
|
| 15-0451 |
PE-Cy5 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) |
488 |
670 |
FC
|
| 17-0451 |
APC anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) |
633 |
660 |
FC
|
| 25-0451 |
Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) |
488 |
760 |
FC
|
| 35-0451 |
PE-Cy5.5 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) |
488 |
690 |
FC
|
| 56-0451 |
Alexa Fluor® 700 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) (APC-Cy5.5 Equivalent) |
633 |
723 |
FC
|
| 45-0451 |
PerCP-Cy5.5 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) (PE-Cy5.5 replacement) |
488 |
690 |
FC
|
| 93-0451 |
eFluorTM 605NC anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) |
350-500 |
605 |
FC
|
| 47-0451 |
Allophycocyanin-eFluorTM 780 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) (APC-Alexa Fluor® 750 Replacement) |
633 |
780 |
FC
|
| 48-0451 |
eFluorTM 450 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) (Pacific Blue® replacement) |
405 |
450 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 30-F11 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The 30-F11 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocyte suspensions. This can be used at less than or equal to 0.03 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Ledbetter, J. A. and L. A. Herzenberg (1979). "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens."
Immunol Rev 47: 63-90.
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Cat. 12-4031 Phycoerythrin (PE) Rat IgG2b Isotype Control
