Product Information

Contents: Phycoerythrin (PE) anti-human CD43 (Leukosialin, Sialophorin)
Catalog Number: 12-0439
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio84-3C1 (84-3C1)
Isotype: Mouse IgG1

Staining of normal human peripheral blood cells with APC anti-human CD3 (UCHT1) (cat. 17-0038) and 0.125 μg of PE Mouse IgG1, K Iso Cntrl (cat. 12-4714) (left) or PE anti-human CD43 (84-3C1) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0439	Affinity Purified anti-human CD43 (Leukosialin, Sialophorin)	N/A	N/A	FC WB
11-0439	Fluorescein isothiocyanate (FITC) anti-human CD43 (Leukosialin, Sialophorin)	488	518	FC
12-0439	Phycoerythrin (PE) anti-human CD43 (Leukosialin, Sialophorin)	488	575	FC
17-0439	Allophycocyanin (APC) anti-human CD43 (Leukosialin, Sialophorin)	633	660	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The monoclonal antibody ebio84-3C1 reacts with CD43, which is also known as leukosialin, galactoglycoprotein and sialophorin. CD43 is a sialomucin which like many mucins can have both adhesive and anti-adhesive functions. Expression of CD43 is found on most leukocytes except resting B lymphocytes. Proteolytic processing upon activation decreases surface expression. CD43 is involved in activation of T cells, B cells, NK cells, and monocytes. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio84-3C1 (84-3C1) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBio84-3C1 (84-3C1) antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood cells. This can be used at 20 μl (0.125 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Borche L, Lozano F, Vilella R, Vives J. CD43 monoclonal antibodies recognize the large sialoglycoprotein of human leukocytes. Eur J Immunol. 1987 Oct;17(10):1523-6.

Schlossman, S., L. Bloumsell, et al. eds. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 12-0431  Phycoerythrin (PE) anti-mouse CD43 (Leukosialin) (clone eBioR2/60)
Cat. 12-0437  Phycoerythrin (PE) anti-human CD43 (Leukosialin, Sialophorin) (clone L10)
Cat. 12-4714  Phycoerythrin (PE) Mouse IgG1, K Isotype Control