Product Information

Contents: Phycoerythrin (PE) anti-human CD42b
Catalog Number: 12-0429
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HIP1
Isotype: Mouse IgG1, κ
HLDA No.: IV P40

PE labeled HIP1 (CD42b) staining on human platelets.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0429	Affinity Purified anti-human CD42b	N/A	N/A	FA FC IHC WB
16-0429	Functional Grade* Purified anti-human CD42b	N/A	N/A	FA FC
11-0429	FITC anti-human CD42b	488	518	FC
12-0429	PE anti-human CD42b	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The HIP1 monoclonal antibody reacts with the human CD42b molecule, a 135 kDa transmembrane protein also known as platelet gpIb.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HIP1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The HIP1 antibody has been pre-titrated and tested by flow cytometric analysis of human platelets. This can be used at 20 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

George N., Q. Wei et al. Staphylcococcus aureus adhesion via Spa, ClfA, and SdrCDE to immobilized platelets demonstrates shear-dependent behavior. 2006. Arterioscler Thromb Vasc Biol 26:2394-2400. (FA, PubMed)

Gohda F., H. Uchiumi et al. 2007. Identification of inherited macrothrombocytopenias based on mean platelet volume among patients diagnosed with idiopathic thrombocytopenia. Thrombosis Research 119:741-746. (FC, PubMed)

Takahashi R., N. Sekine et al. 1999. Influence of Monoclonal Antiplatelet Glycoprotein Antibodies on In Vitro Human Megakaryocyte Colony Formation and Proplatelet Formation. Blood 93(6):1951-1958. (FA, PubMed)

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