Product Information

Contents: Phycoerythrin (PE) anti-mouse CD40
Catalog Number: 12-0401
Sizes: 50 ug, 100 ug, 200 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 1C10
Isotype: Rat IgG2a, κ

Staining of BALB/c splenocytes with 0.25 μg PE Rat IgG2a Iso Cntl (cat. 12-4321) (open histogram) or 0.25 μg PE anti-mouse CD40 (1C10) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0401	Affinity Purified anti-mouse CD40	N/A	N/A	FA FC IP
16-0401	Functional Grade* Purified anti-mouse CD40	N/A	N/A	FC
13-0401	Biotin anti-mouse CD40	N/A	N/A	FC
12-0401	PE anti-mouse CD40	488	575	FC
15-0401	PE-Cy5 anti-mouse CD40	488	670	FC
17-0401	APC anti-mouse CD40	633	660	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 1C10 monoclonal antibody reacts with mouse CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development/maturation by inducing Ig isotype switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 1C10 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The 1C10 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ferlin, W. G., T. von der Weid, et al. (1998). "The induction of a protective response in Leishmania major-infected BALB/c mice with anti-CD40 mAb." Eur J Immunol 28(2): 525-31.
Ferlin, W. G., E. Severinson, et al. (1996). "CD40 signaling induces interleukin-4-independent IgE switching in vivo." Eur J Immunol 26(12): 2911-5.
Heath, A. W., W. W. Wu, et al. (1994). "Monoclonal antibodies to murine CD40 define two distinct functional epitopes." Eur J Immunol 24(8): 1828-34.

Related Products

Cat. 11-0402  FITC anti-mouse CD40 (clone HM40-3)
Cat. 13-0402  Biotin anti-mouse CD40 (clone HM40-3)
Cat. 14-0402  Affinity Purified anti-mouse CD40 (clone HM40-3)
Cat. 16-0402  Functional Grade Purified anti-mouse CD40 (clone HM40-3)
Cat. 12-4321  PE Rat IgG2a Isotype Control