Product Information

Contents: Phycoerythrin (PE) anti-human CD38
Catalog Number: 12-0389
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HIT2
Isotype: Mouse IgG1, κ
HLDA No.: III 155

Surface staining of normal human peripheral blood cells with anti-human CD38 (HIT2) PE . Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0389	Affinity Purified anti-human CD38	N/A	N/A	FC IHC
13-0389	Biotin anti-human CD38	N/A	N/A	FC
11-0389	FITC anti-human CD38	488	518	FC
12-0389	PE anti-human CD38	488	575	FC
15-0389	PE-Cy5 anti-human CD38	488	670	FC
17-0389	Allophycocyanin (APC) anti-human CD38	633	660	FC
25-0389	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD38	488	760	FC
56-0389	Alexa Fluor® 700 anti-human CD38	633	723	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The HIT2 monoclonal antibody reacts with the human CD38 molecule, an approximately 45 kDa type II transmembrane protein. CD38 is expressed by thymocytes, peripheral B cells including plasma cells, activated T cells, and monocytes. CD38 is a counter-receptor for CD31 and has both cyclase and hydrolase enzymatic activity.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HIT2 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The HIT2 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.25 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

McMichael, A.J., P.C.L. Beverly, et al. eds. (1987). Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.
Joshua, D., A. Petersen, et al. (1996). "The labelling index of primitive plasma cells determines the clinical behaviour of patients with myelomatosis." Br J Haematol 94(1): 76-81.
Horenstein, A. L., H. Stockinger, et al. (1998). "CD38 binding to human myeloid cells is mediated by mouse and human CD31." Biochem J 330(Pt 3): 1129-35.
Deaglio, S., M. Morra, et al. (1998). "Human CD38 (ADP-ribosyl cyclase) is a counter-receptor of CD31, an Ig superfamily member." J Immunol 160(1): 395-402.

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