Contents: Phycoerythrin (PE) anti-human CD34 - reacts with Class III epitope on CD34 (Mucosialin)
Catalog Number: 12-0349
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 4H11
Isotype: Mouse IgG1
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Staining of normal human peripheral blood mononuclear cells with FITC anti-human CD45 (HI30) (cat. 11-0459) and PE anti-human CD34 (4H11). Total viable cells were used for analysis.
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Description
The 4H11 monoclonal antibody reacts with human CD34, also known as mucosialin. CD34 belongs to a protein family which also includes endoglycan and podocalyxin. Members of this family are single pass transmembrane proteins with a heavily glycosylated, extracellular, and N-terminal mucin domain. CD34 was first identified as an antigen expressed on hematopoietic progenitors, and has since been extensively used as a marker to isolate cells capable of hematopoietic cell engraftment. In spite of this, the function of CD34 remains unresolved. In addition to expression on hematopoietic progenitors, CD34 is expressed on some populations of mesenchymal stem cells, tumor cell lines and also by vascular endothelia in the adult. Epitopes of CD34 have been assigned to three classes (class I, II or III) based on their differential sensitivity to enzymatic cleavage with neuraminidase, chymopapain and O-glycoprotease. According to this analysis, the 4H11 antibody belongs to class III, indicating that it reacts with a protein epitope.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 4H11 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This 4H11 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood mononuclear cells. This can be used at 20 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).
References
Elknerová K, Lacinová Z, Soucek J, Marinov I, Stöckbauer P. Growth inhibitory effect of the antibody to hematopoietic stem cell antigen CD34 in leukemic cell lines.
Neoplasma. 2007;54(4):311-20. (
4H11, FA, FC,
PubMed)
Sutherland DR, Watt SM, Dowden G, Karhi K, Baker MA, Greaves MF, Smart JE. Structural and partial amino acid sequence analysis of the human hemopoietic progenitor cell antigen CD34.
Leukemia. 1988 Dec;2(12):793-803.
Baumheter S, Singer MS, Henzel W, Hemmerich S, Renz M, Rosen SD, Lasky LA. Binding of L-selectin to the vascular sialomucin CD34.
Science. 1993 Oct 15;262(5132):436-8.
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12-0341 Phycoerythrin (PE) anti-mouse CD34 (clone RAM34)
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12-4714 Phycoerythrin (PE) Mouse IgG1, K Isotype Control
Human Cellular Antigens Chart