PE anti-mouse CD31 antibody: r-phycoerythin (r-PE) conjugated anti-mouse PECAM-1

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Contents: Phycoerythrin (PE) anti-mouse CD31 (PECAM-1)
Catalog Number: 12-0311
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 390
Isotype: Rat IgG2a, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
12-0311-81	50 ug	$100
12-0311-82	100 ug	$195
12-0311-83	200 ug	$280

*International customers: Please contact your distributor for region specific pricing.

Staining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.25 µg PE anti-mouse CD31 (390) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0311	FITC anti-mouse CD31 (PECAM-1)	488	518	FC
12-0311	PE anti-mouse CD31 (PECAM-1)	488	575	FC
13-0311	Biotin anti-mouse CD31 (PECAM-1)	N/A	N/A	FC
14-0311	Affinity Purified anti-mouse CD31 (PECAM-1)	N/A	N/A	FC IH/F IP
16-0311	Functional Grade* Purified anti-mouse CD31 (PECAM-1)	N/A	N/A	FA FC
17-0311	Allophycocyanin (APC) anti-mouse CD31 (PECAM-1)	633	660	FC
25-0311	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD31 (PECAM-1)	488	760	FC
36-0311	Functional Grade* Biotin anti-mouse CD31 (PECAM-1)	N/A	N/A	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 390 monoclonal antibody reacts with mouse CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. This 130-140 kDa surface protein is expressed by endothelial cells and at low levels on all leukocytes and platelets. It has been reported that CD38 binds to CD31. Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interaction, and signal transduction.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 390 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The 390 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Baldwin, H. S., H. M. Shen, et al. 1994. Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31): alternatively spliced, functionally distinct isoforms expressed during mammalian cardiovascular development. Development 120(9): 2539-53.
Horenstein, A. L., H. Stockinger, et al. 1998. CD38 binding to human myeloid cells is mediated by mouse and human CD31. Biochem J 330(Pt 3): 1129-35
Wiewrodt R, Anu P. Thomas, Luca Cipelletti, Melpo Christofidou-Solomidou, David A. Weitz, Sheldon I. Feinstein, David Schaffer, Steven M. Albelda, Michael Koval, and Vladimir R. Muzykantov. 2002. Size-dependent intracellular immunotargeting of therapeutic cargoes into endothelial cells. Blood. 99: 912 - 922.

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