Product Information

Contents: Phycoerythrin (PE) anti-human CD29 (Integrin beta 1)
Catalog Number: 12-0299
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: TS2/16
Isotype: Mouse IgG1

Staining of normal human peripheral blood cells with 0.125 μg of PE Mouse IgG1, K Iso Cntrl (cat. 12-4714) (open histogram) or PE anti-human CD29 (TS2/16) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0299	Affinity Purified anti-human CD29 (Integrin beta 1)	N/A	N/A	FA FC IHC IP
11-0299	Fluorescein isothiocyanate (FITC) anti-human CD29 (Integrin beta 1)	488	518	FC
12-0299	Phycoerythrin (PE) anti-human CD29 (Integrin beta 1)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The TS2/16 monoclonal antibody reacts with human CD29, also known as integrin β1, an approximately 130 kDa single-pass transmembrane glycoprotein. CD29 complexes with one of nine integrin α subunits to form the very late antigen (VLA) subfamily of adhesion molecules. Integrin heterodimers containing CD29 are involved in cell-cell and cell-matrix adhesion. CD29 is expressed broadly on lymphocytes and monocytes, with lower levels of expression on granulocytes. The TS2/16 antibody has been found to possess activating activity for β1 integrins.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This TS2/16 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This TS2/16 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μl (0.125 μg) per million cells in a 100 μl total staining volume.

References

Weber C, Alon R, Moser B, Springer TA. Sequential regulation of α4β1 and α5β1 integrin avidity by CC chemokines in monocytes: implications for transendothelial chemotaxis. J Cell Biol. 1996 Aug;134(4):1063-73. (TS2/16, FA, PubMed)

Hemler ME, Sanchez-Madrid F, Flotte TJ, Krensky AM, Burakoff SJ, Bhan AK, Springer TA, Strominger JL. Glycoproteins of 210,000 and 130,000 M.W. on activated T cells: cell distribution and antigenic relation to components on resting cells and T cell lines. J Immunol. 1984 Jun;132(6):3011-8. (TS2/16, IP, IHC, PubMed)

Sanchez-Madrid F, Krensky AM, Ware CF, Robbins E, Strominger JL, Burakoff SJ, Springer TA. Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3. Proc Natl Acad Sci U S A. 1982 Dec;79(23):7489-93. (TS2/16, IP, FA, PubMed)

Related Products

Cat. 12-0291  Phycoerythrin (PE) anti-mouse CD29 (Integrin b1 chain, a4b1) (clone eBioHMb1-1 (HMb1-1))
Cat. 12-0297  Phycoerythrin (PE) anti-human CD29 (Integrin beta 1) (clone MEM-101A)
Cat. 12-4714  Phycoerythrin (PE) Mouse IgG1, K Isotype Control