Product Information

Contents: Phycoerythrin (PE) anti-rat CD28
Catalog Number: 12-0280
Sizes: 50 ug, 200 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: JJ319
Isotype: Mouse IgG1

Staining of Lou Rat splenocytes with FITC anti-rat CD5 (HIS47) (cat. 11-0050) and 0.25 μg PE Mouse IgG1, K Iso Cntrl (cat. 12-4719) (left) or 0.25 μg PE anti-rat CD28 (JJ319) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0280	Affinity Purified anti-rat CD28	N/A	N/A	FA FC IP
16-0280	Functional Grade* Purified anti-rat CD28	N/A	N/A	FA FC
11-0280	Fluorescein (FITC) anti-rat CD28	488	518	FC
12-0280	Phycoerythrin (PE) anti-rat CD28	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The JJ319 monoclonal antibody reacts with the rat CD28 molecule, expressed by thymocytes, mature T cells, and a subset of NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The JJ319 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This JJ319 antibody has been tested by flow cytometric analysis of rat splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Mitnacht, R., M. Tacke, T. Hunig. 1995. Expression of cell interaction molecules by immature rat thymocytes during passage through the CD4+8+ compartment: developmental regulation and induction by T cell receptor engagement of CD2, CD5, CD28, CD11a, CD44, and CD53. Eur J Immunol. 25:328-32.
Tacke, M., G. Hanke, T. Hanke, T. Hunig. 1997. CD28-mediated induction of proliferation in resting T cells in vitro and in vivo without engagement of the T cell receptor: evidence for functionally distinct forms of CD28. Eur J Immunol. 27:239-47.
Tacke, M., G.J. Clark, M.J. Dallman, T Hunig. 1995. Cellular distribution and costimulatory function of rat CD28. Regulated expression during thymocyte maturation and induction of cyclosporine A sensitivity of costimulated T cell responses by phorbol ester. J Immunol. 154:5121-27.
Bluestone, J.A. 1995. New perspectives of CD28-B7-mediated T cell costimulation. Immunity. 2:555-59.

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Cat. 12-4714 Phycoerythrin (PE) Mouse IgG1, K Isotype Control