Also known as: Interleukin-2 Receptor alpha, IL-2Ra, IL2Ra
RUO: For Research Use Only
Staining of normal human peripheral blood cells with Anti-Human CD4 APC (cat. 17-0049) and 0.06 μg of Mouse IgG2bκ Isotype Control PE (cat. 12-4732) (left) or Anti-Human/Non-Human Primate CD25 PE (right). Cells in the lymphocyte gate were used for analysis.
Contents: Anti-Human/Non-Human Primate CD25 PE Catalog Number: 12-0257 Concentration: 5 µl (0.06 µg)/test Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: CD25-4E3 Host/Isotype: Mouse IgG2b
Product Options
Cat. No.
Size
Price
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12-0257-41
25 tests
12-0257-42
100 tests
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
This CD25-4E3 monoclonal antibody reacts to human CD25, which is also known as the low-affinity interleukin (IL)-2 receptor α. CD25 is expressed on activated T cells (including a subset of regulatory T cells), B cells, and macrophages. This receptor subunit associates with CD122, the IL-2 receptor β chain, and CD132, the common γ chain, to form the high-affinity IL-2 receptor. IL-2 receptor signaling has been linked to cell proliferation and survival in lymphocytes. CD25 is a marker for Hodgkin’s disease, and has been associated with such autoimmune diseases as Type I diabetes.
This monoclonal antibody is cross-reactive to rhesus monkey.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This CD25-4E3 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This CD25-4E3 antibody has been pre-titrated and tested by flow cytometric analysis of normal peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
References
Wang J, Wicker LS, Santamaria P. IL-2 and its high-affinity receptor: genetic control of immunoregulation and autoimmunity. Semin Immunol. 2009 Dec;21(6):363-71.
Engert A, Martin G, Amlot P, Wijdenes J, Diehl V, Thorpe P. Immunotoxins constructed with anti-CD25 monoclonal antibodies and deglycosylated ricin A-chain have potent anti-tumour effects against human Hodgkin cells in vitro and solid Hodgkin tumours in mice. Int J Cancer. 1991 Sep 30;49(3):450-6. (CD25-4E3)
