Contents: Phycoerythrin (PE) anti-human CD21 (CR2)
Catalog Number: 12-0217
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: BU32 (BU-32)
Isotype: Mouse IgG1
HLDA No.: IV
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Surface staining of normal human peripheral blood cells with anti-human CD21 (BU32) PE. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.
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Description
The BU32 monoclonal antibody reacts with human CD21, an approximately 145 kDa type I transmembrane protein. CD21 is expressed by mature B cells, folliculate dendritic cells and a subset of epithelial cells and is a receptor for complement component C3d and Epstein-Barr virus (EBV). CD21, in association with CD19 and CD81, participates in the multimolecular complex with BCR and is involved in B cell activation.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The BU32 (BU-32) antibody has been reported for use in flow cytometric analysis.
Applications Tested
The BU32 (BU-32) antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 5 μl (0.35 μg) per 100μl blood (or per 1 million cells in 100 μl total staining volume).
References
Knapp, W., B. Dorken, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
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12-4714 Phycoerythrin (PE) Mouse IgG1, K Isotype Control
Human Cellular Antigens Chart