Staining of BALB/c splenocytes with Alexa FluorŪ 647 anti-mouse CD19 (1D3) (cat. 51-0193) and 0.125 μg of PE Rat IgG2a Isotype control (cat. 12-4321) (left) or 0.125 μg PE anti-mouse CD20 (AISB12) (right). Cells in the lymphocyte gate were used for analysis.
Contents: Phycoerythrin (PE) anti-mouse CD20 Catalog Number: 12-0201 Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Clone: AISB12 Host/Isotype: Rat IgG2a Storage Conditions: Store at 4°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL.
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Cat. No.
Size
Price
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12-0201-80
25 ug
12-0201-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
CD20 is a B cell-specific 32kDa membrane protein involved in intracellular calcium regulation. CD20 expression begins after the induction of CD19 during development, and is coexpressed with IgM during the pre-B to immature B cell transition in bone marrow. Expression then increases during maturation with almost all mature cells expressing some level of CD20. The human CD20 antigen has proven to be an effective immunotherapeutic target in B cell lymphoma and several autoimmune diseases including rheumatoid arthritis and type I diabetes. CD20 consists of 4 transmembrane domains and a 44 amino acid extracellular loop domain where AISB12 binds.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This AISB12 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This AISB12 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Uchida J, Hamaguchi Y, Oliver JA, Ravetch JV, Poe JC, Haas KM, Tedder TF.The innate mononuclear phagocyte network depletes B lymphocytes through Fc receptor-dependent mechanisms during anti-CD20 antibody immunotherapy.J Exp Med. 2004 Jun 21;199(12):1659-69.
Uchida J, Lee Y, Hasegawa M, Liang Y, Bradney A, Oliver JA, Bowen K, Steeber DA, Haas KM, Poe JC, Tedder TF.Mouse CD20 expression and function.Int Immunol. 2004 Jan;16(1):119-29.
Tedder TF, Klejman G, Disteche CM, Adler DA, Schlossman SF, Saito H.Cloning of a complementary DNA encoding a new mouse B lymphocyte differentiation antigen, homologous to the human B1 (CD20) antigen, and localization of the gene to chromosome 19.J Immunol. 1988 Dec 15;141(12):4388-94.
