PE anti-human CD11a (Integrin alpha L, LFA-1 alpha) antibody: r-phycoerythin (r-PE) conjugate

Contents: Phycoerythrin (PE) anti-human CD11a (LFA-1α)
Catalog Number: 12-0119
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HI111
Isotype: Mouse IgG1, κ
HLDA No.: IV N231

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
12-0119-71	25 tests	$100
12-0119-73	100 tests	$220

*International customers: Please contact your distributor for region specific pricing.

Surface staining of normal human peripheral blood cells with anti-human CD11a (HI111) FITC (left), and PE (right). Autofluorescence is indicated by open histogram. Cells in the lymphocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0119	Affinity Purified anti-human CD11a	N/A	N/A	FA FC IH/F
16-0119	Functional Grade* Purified anti-human CD11a	N/A	N/A	FA FC
11-0119	FITC anti-human CD11a	488	518	FC
12-0119	PE anti-human CD11a	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The HI111 monoclonal antibody reacts with human CD11a, the 180 kDa integrin α_L, also known as the lymphocyte function associated antigen-1 (LFA-1) α chain. LFA-1, formed by non-covalent association of CD11a with CD18 (integrin β₂), serves as an important adhesion molecule involved in lymphocyte and granulocyte function. CD54 (ICAM-1), CD102 (ICAM-2), and CD50 (ICAM-3) are ligands for LFA-1. CD11a is expressed by all leukocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HI111 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The HI111 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (1 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Kapsogeorgou EK, Dimitriou ID, Abu-Helu RF, Moutsopoulos HM, Manoussakis MN. Activation of epithelial and myoepithelial cells in the salivary glands of patients with Sjögren's syndrome: high expression of intercellular adhesion molecule-1 (ICAM.1) in biopsy specimens and cultured cells. Clin Exp Immunol. 2001 Apr;124(1):126-33. (HI111, IHC frozen)

Knapp, W., B. Dorken, et al. eds 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

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