Product Information

Contents: Phycoerythrin (PE) anti-mouse CD11b (Integrin α_M, Mac-1α)
Catalog Number: 12-0112
Sizes: 50 ug, 100 ug, 200 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: M1/70
Isotype: Rat IgG2b, κ

Staining of BALB/c bone marrow cells with PE rat IgG2b isotype control (cat.12-4331) (open histogram) or 0.06μg PE anti-mouse CD11b (M1/70) (colored histogram). Cells in the lymphocyte (left) and myeloid (right) gates were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0112	FITC anti-mouse CD11b (Integrin alpha M, Mac-1 alpha)	488	518	FC
12-0112	PE anti-mouse CD11b (Integrin alpha M, Mac-1 alpha)	488	575	FC
13-0112	Biotin anti-mouse CD11b (Integrin alpha M, Mac-1 alpha)	N/A	N/A	FC
14-0112	Affinity Purified anti-mouse CD11b (Integrin alpha M, Mac-1 alpha)	N/A	N/A	FA FC IH/F IHC(Paraffin) IP
15-0112	PE-Cy5 anti-mouse CD11b (Integrin alpha M, Mac-1 alpha)	488	670	FC
16-0112	Functional Grade* Purified anti-mouse CD11b (Integrin alpha M, Mac-1 alpha)	N/A	N/A	FA FC
17-0112	APC anti-mouse CD11b (Integrin alpha M, Mac-1 alpha)	633	660	FC
19-0112	Cy5 anti-mouse CD11b (Integrin alpha M, Mac-1 alpha)	633	670	FC
25-0112	PE-Cy7 anti-mouse CD11b (Integrin alpha M, Mac-1 alpha)	488	760	FC
27-0112	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD11b (Integrin alpha M, Mac-1 alpha) (APC-Cy7 Equivalent)	633	779	FC
36-0112	Functional Grade* Biotin anti-mouse CD11b (Integrin alpha M, Mac-1 alpha)	N/A	N/A	FA FC IHC IP
45-0112	PerCP-Cy5.5 anti-mouse CD11b (Integrin alpha M, Mac-1 alpha)	488	690	FC
51-0112	Alexa Fluor® 647 anti-mouse CD11b (Integrin alpha M, Mac-1 alpha)	633	668	FC
53-0112	Alexa Fluor® 488 anti-mouse CD11b (Integrin alpha M, Mac-1 alpha)	488	519	FC
56-0112	Alexa Fluor® 700 anti-mouse CD11b (Integrin alpha M, Mac-1 alpha) (APC-Cy5.5 Equivalent)	633	723	FC
57-0112	Pacific Blue® anti-mouse CD11b (Integrin alpha M, Mac-1 alpha)	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The M1/70 monoclonal antibody reacts with mouse CD11b, the 165-170 kDa integrin α_M. CD11b non-covalently associates with CD18 to form α_Mβ₂ integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen. Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes and mouse B-1 cells in the peritoneal cavity. M1/70 is also cross-reactive to human CD11b, and can be used for the detection of this antigen on human peripheral blood monocytes, granulocytes, and a subset of NK cells. Through interactions with its ligands, CD11b participates in adhesive cell interactions.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The M1/70 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The M1/70 antibody has been tested by flow cytometric analysis of mouse splenocyte or bone marrow cell suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

\Springer, T., G. Galfre, et al. 1978. Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur J Immunol 8(8): 539-51.

Springer, T., G. Galfre, et al. 1979. Mac-1: a macrophage differentiation antigen identified by monoclonal antibody. Eur J Immunol 9(4): 301-6.

Sanchez-Madrid, F., P. Simon, et al. 1983. Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1. J Exp Med 158(2): 586-602.

Ault KA and Springer TA. 1981. Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells. J Immunol. 126(1):359-64. (cross-reactivity to human, PubMed)

Dembic Z, Schenck K, and Bogen B. 2000. Dendritic cells purified from myeloma are primed with tumor-specific antigen (idiotype) and activate CD4+ T cells. Proc Natl Acad Sci U S A. 97(6):2697-702. (IHC frozen, PubMed)

Zhang Y, McCormick LL, et al. 2002. Murine sclerodermatous graft-versus-host disease, a model for human scleroderma: cutaneous cytokines, chemokines, and immune cell activation. J Immunol. 168(6):3088-98. (IHC frozen, PubMed)

Whiteland JL, Nicholls SM, et al. 1995. Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem. 43(3):313-20. (IHC paraffin, PubMed)

Related Products

Cat. 11-0117 NEW CATALOG#, SEE 11-0112, FITC anti-m/h CD11b (integrin alpha M, Mac-1 alpha) (clone M1/70)
Cat. 12-4031 Phycoerythrin (PE) Rat IgG2b Isotype Control