Contents: Phycoerythrin (PE) anti-mouse CD11a (Integrin αL, LFA-1α)
Catalog Number: 12-0111
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: M17/4
Isotype: Rat IgG2a, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
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Staining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.25 μg PE anti-mouse CD11a (M17/4) (colored histogram). Total viable cells were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-0111 |
Affinity Purified anti-mouse CD11a (Integrin alpha L, LFA-1 alpha) |
N/A |
N/A |
FA
FC
IHC
IP
|
| 16-0111 |
Functional Grade* Purified anti-mouse CD11a (Integrin alpha L, LFA-1 alpha) |
N/A |
N/A |
FC
|
| 13-0111 |
Biotin anti-mouse CD11a (Integrin alpha L, LFA-1 alpha) |
N/A |
N/A |
FC
|
| 11-0111 |
FITC anti-mouse CD11a (Integrin alpha L, LFA-1 alpha) |
488 |
518 |
FC
|
| 12-0111 |
PE anti-mouse CD11a (Integrin alpha L, LFA-1 alpha) |
488 |
575 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The M17/4 monoclonal antibody reacts with mouse CD11a, the 180 kDa integrin α
L, also known as the lymphocyte function associated antigen-1 (LFA-1) α chain. LFA-1, formed by non-covalent association of CD11a with CD18 (integrin β
2), serves as an important adhesion molecule involved in lymphocyte and granulocyte function. CD54 (ICAM-1), CD102 (ICAM-2), and CD50 (ICAM-3) are ligands for LFA-1. CD11a is expressed by all leukocytes.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The M17/4 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The M17/4 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Sanchez-Madrid, F., P. Simon, et al. 1983. Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1. J Exp Med 158(2): 586-602.
Sanchez-Madrid, F., D. Davignon, et al. 1982. Antigens involved in mouse cytolytic T-lymphocyte (CTL)-mediated killing: functional screening and topographic relationship. Cell Immunol 73(1): 1-11.
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