Product Information

Contents: Phycoerythrin (PE) anti-mouse CD9
Catalog Number: 12-0091
Sizes: 50 ug, 100 ug, 200 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioKMC8 (KMC8)
Isotype: Rat IgG2a, κ

Staining of C57Bl/6 bone marrow cells with FITC anti-mouse Gr-1 (RB6-8C5) (cat. 11-5931) and 0.25 μg of PE Rat IgG2a Iso Cntrl (cat. 12-4321) (left) or PE anti-mouse CD9 (KMC8) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0091	Affinity Purified anti-mouse CD9	N/A	N/A	FC WB
11-0091	Fluorescein isothiocyanate (FITC) anti-mouse CD9	488	518	FC
12-0091	Phycoerythrin (PE) anti-mouse CD9	488	575	FC
51-0091	Alexa Fluor® 647 anti-mouse CD9	633	668	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioKMC8 monoclonal antibody reacts with mouse CD9, a 24 kDa member of the transmembrane 4 superfamily. This family is characterized by the presence of four hydrophobic domains spanning the cell membrane and short N- and C-terminal cytoplasmic domains. CD9 is expressed by several cell types including monocytes, macrophages, platelets, early B cells, activated B and T cells, dendritic cells, eosinophils, basophils, endothelial cells, myoblasts and neuroblasts. On T cells, CD9 functions as a co-stimulatory molecule on naïve T cells. Furthermore, CD9 is expressed in oocytes, and CD9-deficiency results in sterility caused by defective gamete fusion. In mouse macrophages, CD9 functionally associates with FcγRs to modify signals for phagocytosis and inflammatory responses. In mouse B cells, it was discovered that CD9 is a marker for marginal zone B cells, B1 cells, and plasma cells. In dendritic cells, recently it was demonstrated that CD9 facilitates the association of heterologous MHC II molecules. The level of CD9 expression is subject to donor variability.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioKMC8 (KMC8) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBioKMC8 (KMC8) antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Miyake K, Medina KL, Hayashi S, Ono S, Hamaoka T, Kincade PW. Related Articles, Links Monoclonal antibodies to Pgp-1/CD44 block lympho-hemopoiesis in long-term bone marrow cultures. J Exp Med. 1990 Feb 1;171(2):477-88. (KMC8, mAb development, PubMed)

Oritani K, Wu X, Medina K, Hudson J, Miyake K, Gimble JM, Burstein SA, Kincade PW. Antibody ligation of CD9 modifies production of myeloid cells in long-term cultures. Blood. 1996 Mar 15;87(6):2252-61.

Tachibana I, Hemler ME. Role of transmembrane 4 superfamily (TM4SF) proteins CD9 and CD81 in muscle cell fusion and myotube maintenance. J Cell Biol. 1999 Aug 23;146(4):893-904. (KMC8, WB, IP, FA, PubMed)

Related Products

Cat. 12-0098 Phycoerythrin (PE) anti-human CD9 (clone eBioSN4 (SN4 C3-3A2))
Cat. 12-4321 PE Rat IgG2a Isotype Control