Contents: Phycoerythrin (PE) anti-human CD8
Catalog Number: 12-0086
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: OKT8 (OKT-8)
Isotype: Mouse IgG2a
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Staining of normal human peripheral blood cells with PE Mouse IgG2a, K Iso Cntrl (cat. 12-4724) (open histogram) or PE anti-human CD8 (OKT8) (colored histogram). Cells in the lymphocyte gate were used for analysis.
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Description
The OKT8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 β chain (CD8 αβ) or as a homodimer (CD8 αα). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells. Preliminary testing indicates that OKT8 and two other mouse anti-human CD8 antibodies (clone RPA-T8, Cat. No.
14-0088 and clone HIT8a, Cat. No.
14-0089) do not compete with each other for binding to human peripheral blood leukocytes by flow cytometric analysis, suggesting that they do not bind to similar epitopes or block each other by steric hindrance.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This OKT8 (OKT-8) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This OKT8 (OKT-8) antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.03 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).
References
Kay HD, Horwitz DA. 1980. Evidence by reactivity with hybridoma antibodies for a probable myeloid origin of peripheral blood cells active in natural cytotoxicity and antibody-dependent cell-mediated cytotoxicity. J Clin Invest. 66(4):847-51.
Sayos J, Wu C, Morra M, Wang N, Zhang X, Allen D, van Schaik S, Notarangelo L, Geha R, Roncarolo MG, Oettgen H, De Vries JE, Aversa G, Terhorst C.The X-linked lymphoproliferative-disease gene product SAP regulates signals induced through the co-receptor SLAM. Nature. 1998 Oct 1;395(6701):462-9. (
OKT8, IP,
PubMed)
Thomas Y, Sosman J, Irigoyen O, Friedman SM, Kung PC, Goldstein G, Chess L. 1980. Functional analysis of human T cell subsets defined by monoclonal antibodies. I. Collaborative T-T interactions in the immunoregulation of B cell differentiation. J Immunol. 125(6):2402-8.
Campanelli R, Palermo B, Garbelli S, Mantovani S, Lucchi P, Necker A, Lantelme E, Giachino C. 2002. Human CD8 co-receptor is strictly involved in MHC-peptide tetramer-TCR binding and T cell activation. Int Immunol. 14(1):39-44.
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Cat.
12-4724 Phycoerythrin (PE) Mouse IgG2a, K Isotype Control
Human Cellular Antigens Chart