Product Information

Contents: Phycoerythrin (PE) anti-rat CD8a
Catalog Number: 12-0084
Sizes: 25 ug, 100 ug
Formulation: aqeuous buffer, 0.09% sodium azide, contains stabilizer if necessary
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: OX8
Isotype: Mouse IgG1

Staining of rat splenocytes with FITC anti-rat CD3 (G4.18) (cat. 11-0030) and 0.06 μg of PE Mouse IgG1, K Iso Cntrl (cat. 12-4714) (left) or 0.06 μg of PE anti-rat CD8a (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0084	Affinity Purified anti-rat CD8a	N/A	N/A	FA FC IH/F IHC(Paraffin)
12-0084	Phycoerythrin (PE) anti-rat CD8a	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The OX8 monoclonal antibody reacts with rat CD8α. CD8α is a member of the immunoglobulin superfamily which heterodimerizes with CD8β to form the CD8 antigen expressed on CD4+CD8+, double-positive thymocytes, the majority of MHC class I-restricted peripheral T cells, and on some macrophages. CD8 binds to MHC class I expressed on the surface of antigen-presenting cells during antigen presentation, and participates in T-cell receptor signal transduction through association with the kinase Lck.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This OX8 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This OX8 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Johnson P, Gagnon J, Barclay AN, Williams AF. Purification, chain separation and sequence of the MRC OX-8 antigen, a marker of rat cytotoxic T lymphocytes. EMBO J. 1985 Oct;4(10):2539-45. (OX8, protein purification, PubMed)

May E, Dorris ML, Satumtira N, Iqbal I, Rehman MI, Lightfoot E, Taurog JD. CD8 alpha beta T cells are not essential to the pathogenesis of arthritis or colitis in HLA-B27 transgenic rats. J Immunol. 2003 Jan 15;170(2):1099-105. (OX8, FC, FA, PubMed)

Related Products

Cat. 17-0040  Allophycocyanin (APC) anti-rat CD4 (clone OX35)
Cat. 12-0086  Phycoerythrin (PE) anti-human CD8 (clone OKT8 (OKT-8))
Cat. 12-4714  Phycoerythrin (PE) Mouse IgG1, K Isotype Control