Product Information

Contents: Phycoerythrin (PE) anti-mouse CD8 (beta subunit; CD8b, Ly-3)
Catalog Number: 12-0083
Sizes: 50 ug, 100 ug, 200 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioH35-17.2 (H35-17.2)
Isotype: Rat IgG2b, κ

Staining of C57Bl/6 splenocytes with FITC anti-mouse CD8a (53-6.7) (cat. 11-0081) and 0.06 μg of PE Rat IgG2b Isotype Control (cat. 12-4031) (left) or PE anti-mouse CD8b (H35-17.2) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0083	Affinity Purified anti-mouse CD8 (beta subunit; CD8b, Ly-3)	N/A	N/A	FC IH/F IHC(Paraffin) IP
13-0083	Biotin anti-mouse CD8 (beta subunit; CD8b, Ly-3)	N/A	N/A	FC
11-0083	Fluorescein isothiocyanate (FITC) anti-mouse CD8 (beta subunit; CD8b, Ly-3)	488	518	FC
12-0083	Phycoerythrin (PE) anti-mouse CD8 (beta subunit; CD8b, Ly-3)	488	575	FC
15-0083	Phycoerythrin-Cy5 (PE-Cy5) anti-mouse CD8 (beta subunit; CD8b, Ly-3)	488	670	FC
17-0083	Allophycocyanin (APC) anti-mouse CD8 (beta subunit; CD8b, Ly-3)	633	660	FC
53-0083	Alexa Fluor® 488 anti-mouse CD8 (beta subunit; CD8b, Ly-3)	488	519	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioH35-17.2 monoclonal antibody reacts with the mouse CD8β molecule. The CD8β chain associates with the CD8α chain to form the CD8α/β heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic αβ TCR T cells. CD8 binds to MHC class I and plays a role in T cell development and activation of mature T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioH35-17.2 (H35-17.2) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBioH35-17.2 (H35-17.2) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Lefrancois L. Phenotypic complexity of intraepithelial lymphocytes of the small intestine. J Immunol. 1991 Sep 15;147(6):1746-51. (H35-17.2, FC, PubMed)

Ledbetter JA, Seaman WE, Tsu TT, Herzenberg LA. Lyt-2 and lyt-3 antigens are on two different polypeptide subunits linked by disulfide bonds. Relationship of subunits to T cell cytolytic activity. J Exp Med. 1981 Jun 1;153(6):1503-16.

Golstein P, Goridis C, Schmitt-Verhulst AM, Hayot B, Pierres A, van Agthoven A, Kaufmann Y, Eshhar Z, Pierres M. Lymphoid cell surface interaction structures detected using cytolysis-inhibiting monoclonal antibodies. Immunol Rev. 1982;68:5-42. Review.

Tarleton RL, Sun J, et al. 1994. Depletion of T-cell subpopulations results in exacerbation of myocarditis and parasitism in experimental Chagas' disease. Infect Immun. 62(5):1820-9. (H35-17.2, IHC frozen, PubMed)

Thoma-Uszynski S, Emoto M, and Kaufmann SH. 1997. CD8alphaalpha T cells in lesions of Listeria monocytogenes-infected beta2m-deficient mice. Microb Pathog. 23(2):101-6. (H35-17.2, IHC frozen, PubMed)

Related Products

Cat. 12-0080  Phycoerythrin (PE) anti-rat CD8b (beta subunit; CD8b, Ly-3) (clone eBio341 (341))
Cat. 12-0082  Phycoerythrin (PE) anti-mouse CD8 (beta subunit; CD8b, Ly-3) (clone CT-CD8b)
Cat. 12-4031  Phycoerythrin (PE) Rat IgG2b Isotype Control