Contents: Phycoerythrin (PE) anti-rat CD8b (beta subunit; CD8b, Ly-3)
Catalog Number: 12-0080
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio341 (341)
Isotype: Mouse IgG1, κ
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Staining of Lou rat splenocytes with 0.125 μg of PE Mouse IgG1, K Iso Cntrl (cat. 12-4714) (open histogram) or 0.125 μg of PE anti-rat CD8b (341) (colored histogram). Cells in the lymphocyte gate were used for analysis.
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Description
The eBio341 monoclonal antibody reacts with rat CD8β. CD8β is a member of the immunoglobulin superfamily which heterodimerizes with CD8α to form the CD8 antigen expressed on CD4+CD8+, double-positive thymocytes, the majority of MHC class I-restricted peripheral T cells, and on some macrophages. CD8β expression is absent in athymic mice, which are populated by T-cells expressing CD8α homodimers. This has led to the idea that CD8β expression is dependent on the development of T cells in the thymus. CD8 binds to MHC class I expressed on the surface of antigen-presenting cells during antigen presentation, and participates in T-cell receptor signal transduction through association with the kinase Lck.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBio341 (341) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBio341 (341) antibody has been tested by flow cytometric analysis of rat splenocytes and thymocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Torres-Nagel N, Kraus E, Brown MH, Tiefenthaler G, Mitnacht R, Williams AF, Hunig T.
Differential thymus dependence of rat CD8 isoform expression.
Eur J Immunol. 1992 Nov;22(11):2841-8. (
341, FC, FA
PubMed)
Kuhnlein P, Park JH, Herrmann T, Elbe A, Hunig T.
Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T cell receptor.
J Immunol. 1994 Aug 1;153(3):979-86. (
341, FC,
PubMed)
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