Product Information

Contents: Phycoerythrin (PE) anti-human CD7
Catalog Number: 12-0077
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: CD7-6B7
Isotype: Mouse IgG2a
HLDA No.: IV

Surface staining of normal human peripheral blood cells with anti-human CD7 (CD7-6B7) PE. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

Description

The CD7-6B7 monoclonal antibody reacts with human CD7, a 40 kDa type I transmembrane protein. CD7 is expressed by thymocytes, mature T cells, NK cells and pre-B cells. Signaling through crosslinking of CD7 on the surface of T cells induces calcium ion flux.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The CD7-6B7 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The CD7-6B7 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 5 μl (0.5 μg) per 100μl blood (or per 1 million cells in 100 μl total staining volume).

References

Knapp, W., B. Dorken, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

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