Contents: Phycoerythrin (PE) anti-human CD5
Catalog Number: 12-0059
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: UCHT2
Isotype: Mouse IgG1, κ
HLDA No.: III 518
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Surface staining of normal human peripheral blood cells with anti-human CD5 (UCHT2) FITC. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.
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Description
The UCHT2 monoclonal antibody reacts with human CD5, a 67 kDa protein expressed by a majority of thymocytes and mature T cells and a subset of B cells. Signaling through the CD5 molecule activates T cells and binding of CD5 to its ligand on B cells, CD72, and plays an important role in T-B interaction and proliferation.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The UCHT2 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The UCHT2 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl per 100 μl blood (or per 1 million cells in 100 μl total staining volume).
References
Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
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Human Cellular Antigens Chart